Introducing the new PaintScape platform for direct visualization of the 3D genome in situ in single cells

Learn how you can reveal novel mechanisms of disease by directly visualizing disruptions to genome organization in situ in single cells with the groundbreaking PaintScape™ platform

In this webinar, we introduce the PaintScape System for direct visualization of genome organization (the 3D genome) in situ in single cells. Dr. Jude Dunne will provide an overview of the platform and technology as well as present examples of how PaintScape enables unprecedented in situ study of structural genome organization and disruptions—including chromosomal aberrations, translocations, and ecDNA—opening new avenues for understanding the impact of genome structure on gene regulation and cell dysregulation in disease.

He will also present select examples of PaintScape in situ visualization, identification, and characterization of structural genotypes of single cells and cell subpopulations from samples such as human colorectal carcinoma tissue and human breast cancer cell lines representing different stages of cancer.

BACKGROUND

It takes 1.8m of DNA to hold a full human genome, and all that DNA gets packed into a tiny cell nucleus that's about 5–8 µm in diameter. However, in order to fit all that DNA, there is important order and structure to the resulting chromatin architecture—or genome organization. More importantly, it is now known that genome structure and organization have important impact on the regulation of gene expression and cellular programs and their dysfunction when things go awry. The PaintScape System enables -- for the first time-- the ability to study these structural disruptions and elucidate the concomitant mechanisms of dysregulation in individual cells with spatial context.

EXAMPLES OF BIOLOGICAL INSIGHTS PRESENTED

• In situ visualization, identification, and characterization of structural genotypes of single cells and cell subpopulations from select samples such as:
• human breast cancer cells representing different stages of cancer
• human colorectal carcinoma tissue
• Direct spatial in situ visualization and characterization of cell-to-cell differences in genome organization disruptions such as chromosomal aberrations, translocations, ecDNA formation, multi-loci genomic region proximities, focal amplifications, and ploidy changes.

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